Spatial Transcriptomics
Spatial Transcriptomics is a molecular profiling method that determines the cellular localization of mRNA molecules and proteins within a tissue section. This was chosen as the method of the year by Nature magazine in 2020.
The Genomics Core Facility has established different technologies that provide solutions for spatial transcriptomics. These include Visium Spatial 10x Genomics, NanoString’s GeoMx Digital Spatial Profiler, and Element Biosciences AVITI24, for which we are Certified Service Providers.
The Visium Spatial Gene Expression platform delivers unbiased, whole-transcriptome spatial gene expression analysis at the single-cell scale. This platform offers a comprehensive portfolio of assays for studying a wide range of tissues at various resolutions, from Visium v2 at 55 μm to Visium HD with a 2 μm feature size. Visium enables mapping the entire transcriptome on FFPE or fresh-frozen tissue sections to uncover novel insights into normal development and disease biology. Our 10x Genomics Visium CytAssist instrument enables the profiling of pre-sectioned tissues on a standard slide for both FFPE and fresh-frozen spatial workflows.
The GeoMx DSP combines spatial and molecular profiling technologies to generate expression profiles of custom and whole transcriptomes, as well as profiling over 1,000 validated proteins on FFPE or fresh-frozen tissues. With the use of specifiic morphology markers, specififc cell types can be profilled accross the tissue, enabling the study of biology in a spatial context to resolve tissue heterogeneity and the complexity of microenvironments.
The Element Biosciences AVITI24 platform provides spatial and single-cell multiomics, as well as high-quality, affordable sequencing. Using AVITI24, researchers can explore biology at unprecedented depths with subcellular co-detection of RNA, protein, and morphology markers, powered by Avidite Base Chemistry sequencing. Today, AVITI24 offers predesigned and custom gene and protein panels.
For more information about applications, or which technololy is best for you, contact us at Genomics-Core@OUS-research.no.
Technology Overview
Visium Spatial
Element AVITI24
Example from our services (Terrasi, Gillstrøm, Lid, Lorenz, Meza-Zepeda)
Visium Spatial gastrointestinal stromal tumour (GIST)
GeoMx Spatial Transcriptomics of gastrointestinal stromal tumour (GIST)
Morphology marker PanCK (green), CD45 (grey), SYTO13 (blue) & Vimentin (red).
AVITI Spatial Transcriptomics of gastrointestinal stromal tumour (GIST)
News
Summer closure between July 9th and August 8th. The sequencing of existing projects will continue, although turnaround times may be longer than usual.
We wish all our users a good summer
We have performed the first Element Biosciences AVITI24 Teton CytoProfiling run in Europe.
Contact Information:
Genomics-Core@ous-research.no
Daily Leader Core Facility
Susanne Lorenz, PhD
Susanne.Lorenz@ous-research.no
Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.
L.A.Meza-Zepeda@ous-research.no
Follow us via Twitter
Visiting address:
Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building
Norwegian Radium Hospital
Ullernchaussen 66, Lamell 3, 2B
NO-0379 Oslo, NORWAY
+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office
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