The Genomics Core Facility aims to provide researchers with state-of-the-arts high-throughput sequencing and microarray services. As part of our service packages, we provide guidelines to sample preparation and assistance with experimental design.
 
Quantification and quality control of DNA
 
Accurate measurement of DNA quantity and quality is crucial for a successful experiment. High-quality DNA should be high molecular weight and free of contaminants such as carbohydrates, proteins and organic solvents.
 
  • We strongly recommend the use of fluorescent nucleic acid assay for quantification of dsDNA using Quant-iT PicoGreen or Qubit assays.
  • To determine DNA integrity we recommend running a 1% agarose gel electrophoresis or Agilent TapeStation DNA tapescreens.
  • We encourage assessing the purity by spectrophotometer (Nanodrop or equivalent). On the Nanodrop, DNA samples should have an A260/A280 ratio of 1.8 to 2.0, and an A260/A230 ratio higher than 2.0.
  • Samples should be delivered diluted in water or TE buffer.
  • For sample concentration please contact the core facility. Concentration will depend on the protocol.
 
Quantification and quality control of total RNA
 
Precise measurement of RNA quantity and quality is crucial for a successful experiment. RNA with no degradation and free of contaminants such as carbohydrates, proteins and organic solvents is required.
 
  • Total RNA samples should be quantified using the Nanodrop spectrophotometer. RNA samples should have an A260/A280 ratio and A260/A230 of 1.8 to 2.0.
  • All RNA samples deliver for service will be subjected to a quality control using the Agilent 2100 Bioanalyzer prior to downstream processing. The electropherogram and the RNA Integrity Number (RIN) of each sample will be used to assess the RNA integrity. If a sample has a lower RIN value than 8.0, the sample shows low quality and the user should be aware of the occurrence of possible bias in the analysis.
 
Additional costs related to poor sample quality will be responsibility of the user.
 
The Genomics Core Facility recommends the use of Qiagen kits for RNA isolation. RNA should be DNase treated and dissolved in RNase free water. 
 
 
Useful tips on sample preparation and handling

RNA quality control (pdf)

Working with RNA (pdf)

RNA Isolation Using QIAGEN RNeasy Mini Kit

DNA Isolation Using Promega Wizard Kit

 

 
 
 
 


News

Sep 15, 2018

The Genomics Core Facility has recently installed a NanoString nCounter system. The nCounter utilizes a novel digital barcode technology for direct multiplexed measurement of analytes and offers high levels of precision and sensitivity. This technology allows the analysis of expression of up to 800 mRNAs, miRNAs, DNA regions, or proteins simultaneously, using predesigned assays, as well as custom solutions. 

May 5, 2018
The Genomics Core Facility has recently acquired new instrumentation, the ChromiumTM(10X Genomics) and RhapsodyTM(BD Genomics) enabling Single Cell Sequencing and sequencing of Artificial Long Reads.


Contact Information:

Genomics@rr-research.no

Unit Leader Core Facility
Susanne Lorenz, PhD

Susanne.Lorenz@rr-research.no

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

Leonardo.A.Meza-Zepeda@rr-research.no

Visiting address:

Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building 
 
Norwegian Radium Hospital 
Ullernchaussen 66, Lamell 3, 2B 
NO-0379 Oslo, NORWAY

+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office

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