Instrumentation

High-throughtput sequencing

  • HiSeq 2500 - Illumina's latest high capacity high-throughput sequencer with two flow cells running at the same time or independently from each other. Can sequence 5 human whole genome samples with >30x coverage in 10 days. Produces over 600 Gb of sequence per run.
  • MiSeq - The all-in-one personal sequencer from Illumina. Can sequence upto 2 x 250 bp in approx. 24 hours with an output og 7.5-8.5 Gb per run.
  • cBOT - cBot is an automated system that creates clonal clusters from single molecule DNA templates, preparing them for sequencing by synthesis on the HiSeq 2500
  • SciClone NGS workstation - is an automated solution for high-throughput sequencing sample preparation including library prep, sequence capture, and normalization.
  • Agilent Bravo automated liquid handling system for high-throughput seqeuncing. Allows library preparation in 96 well format.
  • Covaris S2 ultrasonicator - The S220 Focused-ultrasonicator is a versatile, high power system engineered for pre-analytical sample processing, such as DNA shearing to specific fragment lengths.
  • LabChipXT - The LabChip XT is an advanced nucleic acid size selection and collection fractionation system that performs automated nucleic acid fractionation where the resulting sample is tightly sized and is delivered in a sequencing compatible buffer.

Microarrays

  • Illumina iScan - BeadArray high-precision scanner that supports an extensive range of array applications. Average scan time per sample (HumanHT-12 v.4 BeadChip) is 2 minutes, 24 minutes for one chip with 12 samples.
  • Illumina AutoLoader 2.x - The AutoLoader 2.x is a device that automatically loads and unloads BeadChip carriers onto a  iScan Reader. 
  • Little Dipper - automated washing robot from SciGene optimised for use with Illumina Expression BeadChips.
  • Tecan liquid handling robot - used with the Illumina methylation and genotyping applications
  • Agilent High Resolution C scanner - a versatile scanner not only for Agilent microarrays, but also for glass microarrays from other providers that match the dimentions specified here. Can scan at from at 10-2 micron resolution.
  • Agilent Microarray hybridization oven - Hybridization Oven designed for optimal hybridization performance and ultimately, consistently reliable data.
  • Affymetrix GeneChip Scanner 3000 7G - The GeneChip® Scanner 3000 7G is an extension of the GeneChip® Scanner 3000 (GCS 3000) series that allows you to scan next-generation higher-density arrays, including SNP arrays with up to 900,000 SNPs, tiling arrays for transcription and all-exon arrays for whole-genome analysis.
  • Affymetrix GeneChip Fluidics Station 450 - used for the wash and stain operation of Affymetrix GeneChip® arrays. We have two Fluidic stations facilitating washing and staining eight GeneChips simultanously.
  • Affymetrix GeneChip Hybridization Oven 645 - provides precise temperature and rotation control to ensure the successful hybridization of as many as 64 GeneChip®-brand cartridges at one time.
  • MAUI hybridization system - For hybridization of Nimblegen arrays. We have two 4-bay hybridization systems.

General

  • Agilent 2100 Bioanalyzer - a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells.
  • Agilent TapeStation 2200 - Automated 96 well format RNA and RNA sample quality control.
  • NanoDrop 1000 - A micro-volume spectrophotometer with fast, direct, easy measurements of DNA, RNA (A260) and protein (A280) concentrations and sample purity (260/280 ratio).
  • Qubit Fluorometer - We have an earlier version of the Qubit 2.0 Fluorometer for quantification of DNA, RNA, and protein.
  • 7900HT Fast Real-Time PCR System - A real-time quantitative PCR system from Applied Biosystems/ Life Technologies

Software

 

 
 
 
 
 
 


News

Feb 24, 2016

The HiSeq 4000 build upon the existing HiSeq 2500 platform using the new HiSeq X patterned flow cell technology, providing unparalleled speed and performance. The dual-flow cell HiSeq 4000 System delivers the highest throughput and lowest price per sample across multiple applications. The new sequencer will provide users will faster turnaround time (run time is 3 days compared to 6-11 days in HiSeq 2500) and higher quality, more data per run and longer reads (150 bp paired-end).

Feb 15, 2016

The Oslo University Hospital Genomics and Bioinformatics Core Facilities will relocate to the new Oslo Cancer Cluster Innovation park to colocalise with the section for Molecular Pathology. The colocalisation will facilitate the technology and competence transfer from our genomics environment to accelerate the implementation of high-throughput sequencing for clinical stratification of cancer patients.


Contact Information:

oslo<at>genomics.no 

Visiting address:
Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building 
 
Norwegian Radium Hospital 
Ullernchaussen 66, Lamell 3, 2B 
NO-0379 Oslo, NORWAY

+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

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