Spatial Transcriptomics

Spatial Transcriptomics is a molecular profiling method to determine the cellular localization of mRNA molecules on a tissue section. This was chosen as the method of the year by Nature magazine in 2020.
 
The Genomics Core Facility has established NanoString’s GeoMx Digital Spatial Profiler and the Visium Spatial 10x Genomics technologies.
 
The GeoMx DSP combines spatial and molecular profiling technologies by generating whole digital transcriptomes and profiling for 100s of validated proteins on FFPE or fresh frozen tissues, enabling to study biology in a spatial context. 
 
The Visium Spatial Gene Expression is a next-generation molecular profiling solution for classifying tissue based on total mRNA. This allows mapping the whole transcriptome with a morphological context in FFPE or fresh frozen tissues to discover novel insights into normal development and disease pathology. The GCF has the only Visium CytAssist instrument in Norway, which enables to profile pre-sectioned tissues using the Visium for FFPE and fresh frozen gene expression workflow.
 
The unique combination of high-plex and high-throughput spatial profiling of these technologies enables researchers to assess and quantify the biological implications of heterogeneity within tissue samples, and to study cellular microenvironments. For more information about applications of these technologies visit NanoString and 10x Genomics websites or contact us at genomics@rr-research.no. 
 

Technology Overview

Visium Spatial 

 

Example from our services

GeoMx Spatial Transcriptomics of gastrointestinal stromal tumour (GIST). Morphology marker PanCK (green), CD45 (grey), SYTO13 (blue) & Vimentin (red).

Visium Spatial gastrointestinal stromal tumour. 

 
 
 
 

News

Jun 1, 2024
Summer Closure 2024

The Genomics Core Facility will not accept new sample submissions over the summer break. The deadline for submission is noon on Friday, July 5th. We will reopen on Tuesday, July 30th. The sequencing of existing projects will continue, although turnaround times may be longer than usual.

We wish all our users a good summer

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May 23, 2024
First Visium HD service

The First Visium HD service was performed this week. High demand for this new technology to study spatial biology at single–cell–scale resolution.

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Contact Information:

Genomics-Core@ous-research.no

Daily Leader Core Facility
Susanne Lorenz, PhD

Susanne.Lorenz@ous-research.no

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

L.A.Meza-Zepeda@ous-research.no

Follow us via Twitter

@OSLOmicsCore

Visiting address:

Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building  
Norwegian Radium Hospital 
Ullernchaussen 66, Lamell 3, 2B 
NO-0379 Oslo, NORWAY

+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office

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