Genomics Core Facility - Single-Cell Analysis

Single-Cell Analysis Platform

The Genomics Core Facility offers a wide variety of single-cell sequencing workflows to study heterogeneity within the epigenome, genome, transcriptome, and proteome at a single-cell resolution. Services are provided using the 10x Genomics Chromium and the BD Rhapsody platform.

These technologies allow the processing of thousands of single cells for downstream molecular analysis. DNA and RNA from every single cell can be barcoded and further investigated using high-throughput sequencing.

We collaborate with our local Flow Cytometry, Advanced Light Microscopy and Bioinformatics Core Facilities at the Institute for Cancer Research to deliver a complete workflow for single-cell analysis.

Applications established for service:

Single-cell 5' & 3’ RNA sequencing: provides quantitative analysis of the whole transcriptome or targeted gene panels using the Chromium or Rhapsody system

Single-cell immune profiling: sequencing of the full V(D)J region for quantitative -cell and B-cell characterization (Chromium & Rhapsody)

Single Cell ATAC: (Assay for Transposase Accessible Chromatin): allows chromatin accessibility profiling of tens of thousands of single cells in parallel

Single-cell multiome: Simultaneously profile gene expression and open chromatin from the same cell with Chromium Single Cell Multiome ATAC + Gene Expression.

Single-cell feature barcoding: Detection of surface protein expression and cell barcoding at the single-cell level.

We are working to establish additional applications: Single nuclei RNA-Seq.

News

Mar 2, 2023

RRBS service now validated on NovaSeq6000

RRBS method (Ovation RRBS Methyl-Seq with TrueMethyl oxBS, Tecan) is now tested and validated on NovaSeq6000 for sequencing offering cheaper methylation analysis

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Jan 4, 2023

Genomics & Bioinformatics resumes Drop-in hour

The Genomics and Bioinformatics Core Facilities resumes the drop-in hour every Wednesday between 2:30-3:30 PM. Follow the Zoom link below.

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Contact Information:

Genomics@rr-research.no

Unit Leader Core Facility
Susanne Lorenz, PhD

Susanne.Lorenz@rr-research.no

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

Leonardo.A.Meza-Zepeda@rr-research.no

Follow us via Twitter

@OSLOmicsCore

Visiting address:

Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building
Norwegian Radium Hospital
Ullernchaussen 66, Lamell 3, 2B
NO-0379 Oslo, NORWAY

+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office

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