The Genomics Core Facility aims to provide researchers with state-of-the-art high-throughput sequencing and microarray services. As part of our service packages, we provide guidelines to sample preparation and assistance with experimental design.
 
Quantification and quality control of DNA
 
Accurate measurement of DNA quantity and quality is crucial for a successful experiment. High-quality DNA should be high molecular weight and free of contaminants such as carbohydrates, proteins, and organic solvents.
 
  • We strongly recommend the use of fluorescent nucleic acid assay for the quantification of dsDNA using Quant-iT PicoGreen or Qubit assays.
  • To determine DNA integrity we recommend running a 1% agarose gel electrophoresis or Agilent TapeStation DNA tapescreens.
  • We encourage assessing purity by spectrophotometer (Nanodrop or equivalent). On the Nanodrop, DNA samples should have an A260/A280 ratio of 1.8 to 2.0, and an A260/A230 ratio higher than 2.0.
  • Samples should be delivered diluted in water or TE buffer.
  • For sample concentration please contact the core facility. Concentration will depend on the protocol.
 
Quantification and quality control of total RNA
 
Precise measurement of RNA quantity and quality is crucial for a successful experiment. RNA with no degradation and free of contaminants such as carbohydrates, proteins, and organic solvents is required.
 
  • Total RNA samples should be quantified using the Nanodrop spectrophotometer. RNA samples should have an A260/A280 ratio and A260/A230 of 1.8 to 2.0.
  • All RNA samples deliver for service will be subjected to quality control using the Agilent 2100 Bioanalyzer prior to downstream processing. The electropherogram and the RNA Integrity Number (RIN) of each sample will be used to assess the RNA integrity. If a sample has a lower RIN value than 8.0, the sample shows low quality and the user should be aware of the occurrence of possible bias in the analysis.
 
Additional costs related to poor sample quality will be the responsibility of the user.
 
The Genomics Core Facility recommends the use of Qiagen kits for RNA isolation. RNA should be DNase treated and dissolved in RNase free water. 
 
 
Useful tips on sample preparation and handling

RNA quality control (pdf)

Working with RNA (pdf)

RNA Isolation Using QIAGEN RNeasy Mini Kit

DNA Isolation Using Promega Wizard Kit

 
 
 
 

News

Dec 3, 2024
New AVITI24 multi-omics sequencer installed

Excited to get the first Element Biosciences AVITI24 instrument installed in Europe. This will strengthen the Genomics Core Facility portfolio of services to support cutting-edge research at OUS, UiO and Norway.

[More]
Jun 1, 2024
Summer Closure 2024

The Genomics Core Facility will not accept new sample submissions over the summer break. The deadline for submission is noon on Friday, July 5th. We will reopen on Tuesday, July 30th. The sequencing of existing projects will continue, although turnaround times may be longer than usual.

We wish all our users a good summer

[More]

Contact Information:

Genomics-Core@ous-research.no

Daily Leader Core Facility
Susanne Lorenz, PhD

Susanne.Lorenz@ous-research.no

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

L.A.Meza-Zepeda@ous-research.no

Follow us via Twitter

@OSLOmicsCore

Visiting address:

Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building  
Norwegian Radium Hospital 
Ullernchaussen 66, Lamell 3, 2B 
NO-0379 Oslo, NORWAY

+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office

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