RRBS service now validated on NovaSeq6000
RRBS method (Ovation RRBS Methyl-Seq with TrueMethyl oxBS, Tecan) is now tested and validated on NovaSeq6000 for sequencing offering cheaper methylation analysis
Reduced representation bisulfite sequencing (RRBS) is an efficient and high-throughput technique for analysing the genome-wide methylation profiles on a single nucleotide level. GCF has offered an RRBS service using the NextSeq500 instrument. We have now validated this assay using the Illumina NovaSeq6000 platform, which decreases sequencing costs for methylation analysis. The Ovation Methyl-Seq workflow combines stagger to eliminate low sequence diversity and increases base quality and UMI’s to account for non-unique reads from the data set. The required input material is 100 ng genomic DNA, from purified high-quality material to FFPE DNA.
News
RRBS method (Ovation RRBS Methyl-Seq with TrueMethyl oxBS, Tecan) is now tested and validated on NovaSeq6000 for sequencing offering cheaper methylation analysis
The Genomics and Bioinformatics Core Facilities resumes the drop-in hour every Wednesday between 2:30-3:30 PM. Follow the Zoom link below.
Contact Information:
Genomics@rr-research.no
Unit Leader Core Facility
Susanne Lorenz, PhD
Susanne.Lorenz@rr-research.no
Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.
Leonardo.A.Meza-Zepeda@rr-research.no
Follow us via Twitter
Visiting address:
Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building
Norwegian Radium Hospital
Ullernchaussen 66, Lamell 3, 2B
NO-0379 Oslo, NORWAY
+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office
................................................................
