May 5, 2018

Single Cell Sequencing and Artificial Long Reads


The Genomics Core Facility has recently acquired new instrumentation, the ChromiumTM(10X Genomics) and RhapsodyTM(BD Genomics) enabling Single Cell Sequencing and sequencing of Artificial Long Reads.
The Genomics Core Facility has recently acquired new instrumentation, the ChromiumTM(10X Genomics) and RhapsodyTM(BD Genomics) enabling Single Cell Sequencing and sequencing of Artificial Long Reads. The equipment allows processing of thousands of single cells for downstream genomic applications. DNA and RNA from each single cell can be barcoded and further investigated using high-throughput sequencing.
 Ana_SC
Applications established for service:
  • 3’ RNA sequencing:quantitative whole transcriptome 3’-end analysis using the ChromiumTMand gene panel (up to 500 genes) analysis using the RhapsodyTMsystem
  • 5’ RNA sequencing:quantitative whole transcriptome analysis also enabeling analysis of transcripts isoforms using different transcription start sites (ChromiumTM
  • V(D)J sequencing:sequencing of the full V(D)J region for quantitative T-cell and B-cell characterization (ChromiumTM)
  • Artificial Long-Read sequencing:Whole genome and exome sequencing combined with linked-reads provides structural and haplotype information from single long molecules of the genome using short-read sequencing (low input required, >1 ng high molecular weight DNA)

 
 
 
 


News

Mar 20, 2024

The Genomics Core Facility has successfully gone through a certification process to become a certified NGS ProLab for TWIST Bioscience technologies. 

Jan 2, 2024

The Genomics Core Facility at Oslo University Hospital and University of Oslo is now a Core Lab for nCounter and GeoMx technologies. 


Contact Information:

Genomics@rr-research.no

Daily Leader Core Facility
Susanne Lorenz, PhD

Susanne.Lorenz@rr-research.no

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

L.A.Meza-Zepeda@ous-research.no

Follow us via Twitter

@OSLOmicsCore

Visiting address:

Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building 
 
Norwegian Radium Hospital 
Ullernchaussen 66, Lamell 3, 2B 
NO-0379 Oslo, NORWAY

+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office

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