May 5, 2018

Single Cell Sequencing and Artificial Long Reads


The Genomics Core Facility has recently acquired new instrumentation, the ChromiumTM(10X Genomics) and RhapsodyTM(BD Genomics) enabling Single Cell Sequencing and sequencing of Artificial Long Reads.
The Genomics Core Facility has recently acquired new instrumentation, the ChromiumTM(10X Genomics) and RhapsodyTM(BD Genomics) enabling Single Cell Sequencing and sequencing of Artificial Long Reads. The equipment allows processing of thousands of single cells for downstream genomic applications. DNA and RNA from each single cell can be barcoded and further investigated using high-throughput sequencing.
 Ana_SC
Applications established for service:
  • 3’ RNA sequencing:quantitative whole transcriptome 3’-end analysis using the ChromiumTMand gene panel (up to 500 genes) analysis using the RhapsodyTMsystem
  • 5’ RNA sequencing:quantitative whole transcriptome analysis also enabeling analysis of transcripts isoforms using different transcription start sites (ChromiumTM
  • V(D)J sequencing:sequencing of the full V(D)J region for quantitative T-cell and B-cell characterization (ChromiumTM)
  • Artificial Long-Read sequencing:Whole genome and exome sequencing combined with linked-reads provides structural and haplotype information from single long molecules of the genome using short-read sequencing (low input required, >1 ng high molecular weight DNA)

 
 
 
 


News

Sep 15, 2018

The Genomics Core Facility has recently installed a NanoString nCounter system. The nCounter utilizes a novel digital barcode technology for direct multiplexed measurement of analytes and offers high levels of precision and sensitivity. This technology allows the analysis of expression of up to 800 mRNAs, miRNAs, DNA regions, or proteins simultaneously, using predesigned assays, as well as custom solutions. 

May 5, 2018
The Genomics Core Facility has recently acquired new instrumentation, the ChromiumTM(10X Genomics) and RhapsodyTM(BD Genomics) enabling Single Cell Sequencing and sequencing of Artificial Long Reads.


Contact Information:

Genomics@rr-research.no

Unit Leader Core Facility
Susanne Lorenz, PhD

Susanne.Lorenz@rr-research.no

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

Leonardo.A.Meza-Zepeda@rr-research.no

Visiting address:

Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building 
 
Norwegian Radium Hospital 
Ullernchaussen 66, Lamell 3, 2B 
NO-0379 Oslo, NORWAY

+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office

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