Dec 2, 2014

The regulatory landscape of osteogenic differentiation.


The article "The regulatory landscape of osteogenic differentiation" publiched by Håkelien AM, in Stemce Cells has been awarded one of teh six excellent articles from the first half of 2014. The entire data set for this paper has been produced at the Genomics Core Facility.
Differentiation of osteoblasts from mesenchymal stem cells (MSCs) is an integral part of bone development and homeostasis, and may when improperly regulated cause disease such as bone cancer or osteoporosis. Using unbiased high-throughput methods we here characterize the landscape of global changes in gene expression, histone modifications, and DNA methylation upon differentiation of human MSCs to the osteogenic lineage. Furthermore, we provide a first genome-wide characterization of DNA binding sites of the bone master regulatory transcription factor Runt-related transcription factor 2 (RUNX2) in human osteoblasts, revealing target genes associated with regulation of proliferation, migration, apoptosis, and with a significant overlap with p53 regulated genes. These findings expand on emerging evidence of a role for RUNX2 in cancer, including bone metastases, and the p53 regulatory network. We further demonstrate that RUNX2 binds to distant regulatory elements, promoters, and with high frequency to gene 3' ends. Finally, we identify TEAD2 and GTF2I as novel regulators of osteogenesis.

 
 
 
 


News

Sep 15, 2018

The Genomics Core Facility has recently installed a NanoString nCounter system. The nCounter utilizes a novel digital barcode technology for direct multiplexed measurement of analytes and offers high levels of precision and sensitivity. This technology allows the analysis of expression of up to 800 mRNAs, miRNAs, DNA regions, or proteins simultaneously, using predesigned assays, as well as custom solutions. 

May 5, 2018
The Genomics Core Facility has recently acquired new instrumentation, the ChromiumTM(10X Genomics) and RhapsodyTM(BD Genomics) enabling Single Cell Sequencing and sequencing of Artificial Long Reads.


Contact Information:

Genomics@rr-research.no

Unit Leader Core Facility
Susanne Lorenz, PhD

Susanne.Lorenz@rr-research.no

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

Leonardo.A.Meza-Zepeda@rr-research.no

Visiting address:

Genomics Core Facility (Room 3-F8-04)
Department of Core facilities
Institute for Cancer Research
New OCCI-building 
 
Norwegian Radium Hospital 
Ullernchaussen 66, Lamell 3, 2B 
NO-0379 Oslo, NORWAY

+47 - 9183 2772 Laboratory
+47 - 9183 3425 Office

................................................................

................................................................