Dec 2, 2014

The regulatory landscape of osteogenic differentiation.


The article "The regulatory landscape of osteogenic differentiation" publiched by Håkelien AM, in Stemce Cells has been awarded one of teh six excellent articles from the first half of 2014. The entire data set for this paper has been produced at the Genomics Core Facility.
Differentiation of osteoblasts from mesenchymal stem cells (MSCs) is an integral part of bone development and homeostasis, and may when improperly regulated cause disease such as bone cancer or osteoporosis. Using unbiased high-throughput methods we here characterize the landscape of global changes in gene expression, histone modifications, and DNA methylation upon differentiation of human MSCs to the osteogenic lineage. Furthermore, we provide a first genome-wide characterization of DNA binding sites of the bone master regulatory transcription factor Runt-related transcription factor 2 (RUNX2) in human osteoblasts, revealing target genes associated with regulation of proliferation, migration, apoptosis, and with a significant overlap with p53 regulated genes. These findings expand on emerging evidence of a role for RUNX2 in cancer, including bone metastases, and the p53 regulatory network. We further demonstrate that RUNX2 binds to distant regulatory elements, promoters, and with high frequency to gene 3' ends. Finally, we identify TEAD2 and GTF2I as novel regulators of osteogenesis.

 
 
 
 
 
 


News

Jan 18, 2018

Due to a resent hacker attack to the OUS IT system, we currently are unable to receive or send emails to the rr-research domain. Until further notice please use this email to contact us “genomics.cf.oslo@gmail.com”

Feb 24, 2016

The HiSeq 4000 build upon the existing HiSeq 2500 platform using the new HiSeq X patterned flow cell technology, providing unparalleled speed and performance. The dual-flow cell HiSeq 4000 System delivers the highest throughput and lowest price per sample across multiple applications. The new sequencer will provide users will faster turnaround time (run time is 3 days compared to 6-11 days in HiSeq 2500) and higher quality, more data per run and longer reads (150 bp paired-end).


Contact Information:

genomics.cf.oslo@gmail.com

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Daily Leader Core Facility
Susanne Lorenz, PhD

Head of Core Facility
Leonardo A. Meza-Zepeda, Dr. philos.

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